Viral Vector Services
AAV Vector Upstream Processes
We assist with selecting the producer cell line and culture type based on the AAV serotype and other factors and needs.
Our optimized E. coli processes produce high-quality AAV plasmids for transfection
The AAV vector upstream processes include:
- Mammalian cell culture, e.g., HEK293, or Sf9 insect cells
- Transfection with plasmids or transduction with baculovirus, depending on the selected approach and serotype
- Cell harvest and lysis
- Analysis of harvest
Suspension cell culture
Suspension cultures can be readily upscaled, as the limiting factor for cell growth is the concentration of cells in the medium. Suspension cells can be cultured in serum-free media, therefore free from bovine-derived impurities such as TSE and BSE.
Adherent cell culture
Adherent cell culture applies to most cell types. Some AAV vector constructs only produce good yields in adherent cultures. Growth monitoring of adherent cell cultures can be carried out visually under the microscope. Adherent cell growth is limited by surface area, which may limit product yields.
With you all the way…
together, making advanced biologics, and tomorrow’s life-transforming therapies.
We deliver unparalleled end-to-end development and manufacturing services for all protein expression systems and viral vectors, both for Drug Substances and Drug Products, from pre-clinical to clinical development and commercial phases.
We support our customers wherever they are on their journey, from early-stage development to commercialization. We are passionate, precise, and always close, together, making advanced biologics and tomorrow’s life-transforming therapies.
Pre-Clinical
Phase I
Phase II
Phase III
Commercial