Flow cytometry is a laser-based analytical technique used to simultaneously measure multiple physical and biological characteristics of individual cells or particles as they pass through a focused light beam in a fluid stream. In cell therapy manufacturing and biopharmaceutical development, it is used for cell counting, viability assessment, immunophenotyping, sterility monitoring, and potency testing. It is a critical quality control tool in the manufacturing of CAR-T cells, stem cells, and other ATMPs, and supports regulatory submissions requiring quantitative cell characterization data.
